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Zephyrin-based microarray |
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 Zephyrin, a key component of Zephyr’s technology, is a unique cellulose-binding
domain (CBD) molecule, involved in targeting and
bindig of polysaccharide- degrading
enzymes, i.e. cellulasaes, onto cellulose in
the bacterium
Clostridium thermocellum.
The selective and specific interaction between Zephyrin and cellulose
creates a universal proprietary platform technology for the generation
of manifold applications mainly in the field of peptide, antibody and
small molecule microarray, as well as, high-throughput
screening
(HTS)
applications. The cellulose-coated slide (cellulo-slide™)
is the second key element in Zephyr microarray technology. This specific
slide consists of 3-dimensional cellulose membrane that provides
exceptional low protein binding feature and high-binding capacity for
the Zephyrin molecule.
Zephyrin-based microarray presents a set of advantages over other technologies currently on the market or in development.
Our Key Selling Points are:
- Rapidity — does not require extraneous purification and/or enrichment steps of the target sample.
- Sensitivity and Specificity — has a high sensitivity in the attomole range with an excellent Signal-to-Noise ratio.
- Accuracy — contains unique internal quality control standard for assessment of printing efficacy.
- Flexibility — simple, fast and efficient mode of fabrication with high stability and flexibility for different biological and chemical moieties.
- Low Cost — user costs of Zephyrin’s–based microarrays are extremely low compared to other alternatives.
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Zephyr|Glossary |
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Zephyrin – Zephyrin is a universal recombinant display molecule exploited for protein microaarry, affinity purification and recombinant expression of peptides and proteins applications. The Zephyrin molecule is belongs to the cellulose-binding domain family and is the major cellulose-binding domain from the cellulosome of the bacterium Clostridium thermocellum. The Zephyrin molecule has been successfully exploited as an affinity tag for expression and purification of variety peptides and proteins.
Cellulose-binding
domain –
Cellulose
binding domains are found in bacteria and fungus as a
discrete domains in hydrolytic proteins, such as in cellulases
that are known for their cellulose-degradation activity.
Recently, cellulose-binding domains have been also identified
in proteins that have no detectable cellulase activity.
In native cellulases, it is thought that cellulose-binding
domains concentrate the catalytic domains on the surface
of the insoluble cellulose substrate and aids their efficient
solubilization. In molecule with no hydrolytic activity,
the cellulose-binding domains are part of a multi-domain
protein called scaffoldin that is involved in integrating
the catalytic subunits into a cohesive multienzyme complex
known as a cellulosome.
High-throughput screening – often abbreviated as HTS, is a method for scientific experimentation especially used in drug discovery and allows a researcher to effectively perform millions of biochemical tests in a short period of time. The HTS process allows the rapid identification of active compounds, antibodies or even genes which modulate a particular biomolecular pathway.
Cellulose – The cellulose is unbranched insoluble homopolymer that is the major constituent of plant matter. Cellulose is remarkably stable and constituting of linear polymer of b-1,4-linked glucose. The individual cellulose chains contain from about 100 to more than 10000 glucose units, packed in parallel, three-dimensional fashion, packed in parallel, three-dimensional fashion into microfibrils. Cellulose is an attractive matrix for affinity purification and for immobi-lization mainly because of its excellent non-specific binding properties. To exploit the characteristics of this matrix, Zephyr investigators have used a protein domain that naturally binds to cellulose and called Zephyrin. |
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